Composite
Part:BBa_K3002230:Design
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 spectinomycin resistance + cCA_WT-PETase_SP20His + cCA_MHETase_SP20HA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5348
Illegal PstI site found at 3485
Illegal PstI site found at 4458
Illegal PstI site found at 6690
Illegal PstI site found at 7014
Illegal PstI site found at 7357
Illegal PstI site found at 8167 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5348
Illegal NheI site found at 2665
Illegal NheI site found at 5612
Illegal PstI site found at 3485
Illegal PstI site found at 4458
Illegal PstI site found at 6690
Illegal PstI site found at 7014
Illegal PstI site found at 7357
Illegal PstI site found at 8167
Illegal NotI site found at 7025 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5348
Illegal BglII site found at 7935 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5348
Illegal PstI site found at 3485
Illegal PstI site found at 4458
Illegal PstI site found at 6690
Illegal PstI site found at 7014
Illegal PstI site found at 7357
Illegal PstI site found at 8167 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5348
Illegal PstI site found at 3485
Illegal PstI site found at 4458
Illegal PstI site found at 6690
Illegal PstI site found at 7014
Illegal PstI site found at 7357
Illegal PstI site found at 8167
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3220
Illegal NgoMIV site found at 3247
Illegal NgoMIV site found at 4949
Illegal NgoMIV site found at 6623 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002124 (cCA_wildtype PETase_SP20His) and BBa_K3002114 (cCA_wildtype MHETase_SP20HA).